欧洲冠军联赛_欧洲冠军联赛投注_官网

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欧洲冠军联赛_欧洲冠军联赛投注_官网

工学部 環境建設工学科

教員紹介

教員プロフィール

宮内 啓介 教授 (環境生物工学)
学位 博士(農学) (東京大学 1999年)
専門 環境微生物工学研究分野
所属学会 日本農芸化学会、日本生物工学会、環境バイオテクノロジー学会、アメリカ微生物学会、土木学会、ゲノム微生物学会
その他 生年月日: 1971年 2月3日
担当科目 生命の科学、環境生物工学、情報リテラシー、自然科学実験ファンダメンタルズ
■経歴
  東京大学農学生命科学研究科応用生命工学専攻博士課程
1999年4月~2000年3月 日本学術振興会特別研究員
2000年4月~2006年3月 長岡技術科学大学助手
2006年4月~2007年3月 欧洲冠军联赛_欧洲冠军联赛投注_官网工学部助教授
2007年4月~2012年3月 助教授から准教授へ名称変更
2012年4月~ 欧洲冠军联赛_欧洲冠军联赛投注_官网工学部教授

■業績

査読つき論文リスト
  • Nagata, Y., R. Ohtomo, K. Miyauchi, M. Fukuda, K. Yano, and M. Takagi. 1994. Cloning and sequencing of a 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase gene involved in the degradation of gamma-hexachlorocyclohexane in Pseudomonas paucimobilis. J Bacteriol 176:3117-25.
  • Nagata, Y., K. Miyauchi, J. Damborsky, K. Manova, A. Ansorgova, and M. Takagi. 1997. Purification and characterization of a haloalkane dehalogenase of a new substrate class from a gamma-hexachlorocyclohexane-degrading bacterium, Sphingomonas paucimobilis UT26. Appl Environ Microbiol 63:3707-10.
  • Miyauchi, K., S. K. Suh, Y. Nagata, and M. Takagi. 1998. Cloning and sequencing of a 2,5-dichlorohydroquinone reductive dehalogenase gene whose product is involved in degradation of gamma-hexachlorocyclohexane by Sphingomonas paucimobilis. J Bacteriol 180:1354-9.
  • Miyauchi, K., Y. Adachi, Y. Nagata, and M. Takagi. 1999. Cloning and sequencing of a novel meta-cleavage dioxygenase gene whose product is involved in degradation of gamma-hexachlorocyclohexane in Sphingomonas paucimobilis. J Bacteriol 181:6712-9.
  • Nagata, Y., A. Futamura, K. Miyauchi, and M. Takagi. 1999. Two different types of dehalogenases, LinA and LinB, involved in gamma-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26 are localized in the periplasmic space without molecular processing. J Bacteriol 181:5409-13.
  • Ohtsubo, Y., K. Miyauchi, K. Kanda, T. Hatta, H. Kiyohara, T. Senda, Y. Nagata, Y. Mitsui, and M. Takagi. 1999. PcpA, which is involved in the degradation of pentachlorophenol in Sphingomonas chlorophenolica ATCC39723, is a novel type of ring-cleavage dioxygenase. FEBS Lett 459:395-8.
  • Nagata, Y., K. Miyauchi, and M. Takagi. 1999. Complete analysis of genes and enzymes for gamma-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26. J Ind Microbiol Biotechnol 23:380-390.
  • Kitagawa, W., K. Miyauchi, E. Masai, and M. Fukuda. 2001. Cloning and characterization of benzoate catabolic genes in the gram-positive polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1. J Bacteriol 183:6598-606.
  • Kitagawa, W., S. Takami, K. Miyauchi, E. Masai, Y. Kamagata, J. M. Tiedje, and M. Fukuda. 2002. Novel 2,4-dichlorophenoxyacetic acid degradation genes from oligotrophic Bradyrhizobium sp. strain HW13 isolated from a pristine environment. J Bacteriol 184:509-18.
  • Lee, H. S., K. Miyauchi, Y. Nagata, R. Fukuda, S. Sasagawa, H. Endoh, S. Kato, H. Horiuchi, M. Takagi, and A. Ohta. 2002. Employment of the human estrogen receptor beta ligand-binding domain and co-activator SRC1 nuclear receptor-binding domain for the construction of a yeast two-hybrid detection system for endocrine disrupters. J Biochem (Tokyo) 131:399-405.
  • Miyauchi, K., H. S. Lee, M. Fukuda, M. Takagi, and Y. Nagata. 2002. Cloning and characterization of linR, involved in regulation of the downstream pathway for gamma-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26. Appl Environ Microbiol 68:1803-7.
  • Sakai, M., K. Miyauchi, N. Kato, E. Masai, and M. Fukuda. 2003. 2-Hydroxypenta-2,4-dienoate metabolic pathway genes in a strong polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1. Appl Environ Microbiol 69:427-33.
  • Masai, E., A. Ichimura, Y. Sato, K. Miyauchi, Y. Katayama, and M. Fukuda. 2003. Roles of the enantioselective glutathione S-transferases in cleavage of beta-aryl ether. J Bacteriol 185:1768-75.
  • Hara, H., E. Masai, K. Miyauchi, Y. Katayama, and M. Fukuda. 2003. Characterization of the 4-carboxy-4-hydroxy-2-oxoadipate aldolase gene and operon structure of the protocatechuate 4,5-cleavage pathway genes in Sphingomonas paucimobilis SYK-6. J Bacteriol 185:41-50.
  • Masai, E., M. Sasaki, Y. Minakawa, T. Abe, T. Sonoki, K. Miyauchi, Y. Katayama, and M. Fukuda. 2004. A novel tetrahydrofolate-dependent O-demethylase gene is essential for growth of Sphingomonas paucimobilis SYK-6 with syringate. J Bacteriol 186:2757-65.
  • Takeda, H., N. Hara, M. Sakai, A. Yamada, K. Miyauchi, E. Masai, and M. Fukuda. 2004. Biphenyl-inducible promoters in a polychlorinated biphenyl-degrading bacterium, Rhodococcus sp. RHA1. Biosci Biotechnol Biochem 68:1249-58.
  • Takeda, H., A. Yamada, K. Miyauchi, E. Masai, and M. Fukuda. 2004. Characterization of transcriptional regulatory genes for biphenyl degradation in Rhodococcus sp. strain RHA1. J Bacteriol 186:2134-46.
  • Warren, R., W. W. Hsiao, H. Kudo, M. Myhre, M. Dosanjh, A. Petrescu, H. Kobayashi, S. Shimizu, K. Miyauchi, E. Masai, G. Yang, J. M. Stott, J. E. Schein, H. Shin, J. Khattra, D. Smailus, Y. S. Butterfield, A. Siddiqui, R. Holt, M. A. Marra, S. J. Jones, W. W. Mohn, F. S. Brinkman, M. Fukuda, J. Davies, and L. D. Eltis. 2004. Functional characterization of a catabolic plasmid from polychlorinated- biphenyl-degrading Rhodococcus sp. strain RHA1. J Bacteriol 186:7783-95.
  • Kasai, D., E. Masai, K. Miyauchi, Y. Katayama, and M. Fukuda. 2004. Characterization of the 3-O-methylgallate dioxygenase gene and evidence of multiple 3-O-methylgallate catabolic pathways in Sphingomonas paucimobilis SYK-6. J Bacteriol 186:4951-9.
  • Sakai, M, E. Masai, K. Sugiyama, C. Takahashi, A. Yamada, K. Miyauchi, and M. Fukuda. 2004. Characterization of a second 2,3-dihydro-2.3-dihydroxybiphenyl dehydrogenase gene for the polychlorinated bipenyl degrader Rhodococcus sp. strain RHA1. J Environ. Biotech. 4(1):41-47.
  • Miyauchi, K., M. Fukuda, M. Tsuda, M. Takagi, and Y. Nagata. 2005. Identification of insertion sequence from a gamma-hexachlorocyclohexane degrading bacterium, Sphingomonas paucimobilis UT26. Biosci Biotechnol Biochem 69:216-9.
  • Kasai, D., E. Masai, K. Miyauchi, Y. Katayama, and M. Fukuda. 2005. Characterization of the gallate dioxygenase gene: three distinct ring cleavage dioxygenases are involved in syringate degradation by Sphingomonas paucimobilis SYK-6. J Bacteriol 187:5067-74.
  • Peng, X., E. Masai, D. Kasai, K. Miyauchi, Y. Katayama, and M. Fukuda. 2005. A second 5-carboxyvanillate decarboxylase gene, ligW2, is important for lignin-related biphenyl catabolism in Sphingomonas paucimobilis SYK-6. Appl Environ Microbiol 71:5014-21.
  • Abe, T., E. Masai, K. Miyauchi, Y. Katayama, and M. Fukuda. 2005. A tetrahydrofolate-dependent O-demethylase, LigM, is crucial for catabolism of vanillate and syringate in Sphingomonas paucimobilis SYK-6. J Bacteriol 187:2030-7.
  • Endo, R., M. Kamakura, K. Miyauchi, M. Fukuda, Y. Ohtsubo, M. Tsuda, and Y. Nagata. 2005. Identification and characterization of genes involved in the downstream degradation pathway of gamma-hexachlorocyclohexane in Sphingomonas paucimobilis UT26. J Bacteriol 187:847-53.
  • Sasoh, M., E. Masai, S. Ishibashi, H. Hara, N. Kamimura, K. Miyauchi, and M. Fukuda. 2006. Characterization of the terephthalate degradation genes of Comamonas sp. strain E6. Appl Environ Microbiol 72:1825-32.
  • Iwasaki T, Miyauchi K, Masai E, Fukuda M. 2006. Multiple-subunit genes of the aromatic-ring-hydroxylating dioxygenase play an active role in biphenyl and polychlorinated biphenyl degradation in Rhodococcus sp. strain RHA1. Appl Environ Microbiol 72:5396-402.
  • McLeod MP, Warren RL, Hsiao WW, Araki N, Myhre M, Fernandes C, Miyazawa D, Wong W, Lillquist AL, Wang D, Dosanjh M, Hara H, Petrescu A, Morin RD, Yang G, Stott JM, Schein JE, Shin H, Smailus D, Siddiqui AS, Marra MA, Jones SJ, Holt R, Brinkman FS, Miyauchi K, Fukuda M, Davies JE, Mohn WW, Eltis LD. 2006. The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse. Proc Natl Acad Sci U S A. 2006 103:15582-7.
  • Iwasaki T, Takeda H, Miyauchi K, Yamada T, Masai E, Fukuda M. 2007. Characterization of two biphenyl dioxygenases for biphenyl/PCB degradation in A PCB degrader, Rhodococcus sp. strain RHA1. Biosci Biotechnol Biochem. 71: 993-1002.
  • Yamada T, Miyauchi K, Ueda H, Ueda Y, Sugawara H, Nakai Y, Endo G. 2007. Composting cattle dung wastes by using a hyperthermophilic pre-treatment process: characterization by physicochemical and molecular biological analysis. J Biosci Bioeng. 104:408-15.
  • 山田剛史,宮内啓介,上田裕一,上田英代,遠藤銀朗. 2007.有機資源の循環利用に必要な環境保全型コンポスト製造技術の開発. 環境バイオテクノロジー学会誌,7:111-117
  • Miyauchi K, Sukda P, Nishida T, Ito E, Matsumoto Y, Masai E, Fukuda M. 2008. Isolation of dibenzofuran-degrading bacterium, Nocardioides sp. DF412, and characterization of its dibenzofuran degradation genes. J Biosci Bioeng. 105:628-35.
  • Yamada T, Suzuki A, Ueda H, Ueda Y, Miyauchi K, Endo G. 2008. Successions of bacterial community in composting cow dung wastes with or without hyperthermophilic pre-treatment. Appl Microbiol Biotechnol. 81:771-81.
  • Sukda P, Gouda N, Ito E, Miyauchi K, Masai E, Fukuda M. 2009. Characterization of a Transcriptional Regulatory Gene Involved in Dibenzofuran Degradation by Nocardioides sp. Strain DF412. Biosci Biotechnol Biochem. 73:508-16
  • Takeda, H., Shimodaira, J., Yukawa, K., Hara, N., Kasai, D., Miyauchi, K., Masai, E., Fukuda, M. 2010. Dual Two-Component Regulatory Systems Are Involved in Aromatic Compound Degradation in a Polychlorinated-Biphenyl Degrader, Rhodococcus jostii RHA1. J Bacteriol. 192:4741-4751
  • Miyahara, M., Kim, S. W., Fushinobu, S., Takaki, K., Yamada, T., Watanabe, A., Miyauchi, K., Endo, G., Wakagi, T., Shoun, H. 2010. Potential of Aerobic Denitrification by Pseudomonas stutzeri TR2 To Reduce Nitrous Oxide Emissions from Wastewater Treatment Plants. Appl Environ Microbiol. 76:4619-4625
  • Ohmori T, Morita H, Tanaka M, Tomoi M, Miyauchi K, Kasai D, Furukawa K, Masai E, Fukuda M. 2011. Expression in Escherichia coli of biphenyl 2,3-dioxygenase genes from a Gram-positive polychlorinated biphenyl degrader, Rhodococcus jostii RHA1. Biosci Biotechnol Biochem. 75:26-33.
  • Araki N, Niikura Y, Miyauchi K, Kasai D, Masai E, Fukuda M. 2011. Glucose-mediated transcriptional repression of PCB/biphenyl catabolic genes in Rhodococcus jostii RHA1. J Mol Microbiol Biotechnol. 20:53-62.
  • Araki N, Suzuki T, Miyauchi K, Kasai D, Masai E, Fukuda M. 2011. Identification and Characterization of Uptake Systems for Glucose and Fructose in Rhodococcus jostii RHA1. J Mol Microbiol Biotechnol. 20:125-136.
  • Ohmori T, Morita H, Tanaka M, Miyauchi K, Kasai D, Furukawa K, Miyashita K, Ogawa N, Masai E, Fukuda M. 2011. Development of a strain for efficient degradation of polychlorinated biphenyls by patchwork assembly of degradation pathways. J Biosci Bioeng. 111:437-442.
  • Nguyen AT, Sato Y, Iwasaki T, Miyauchi K, Tokuda M, Kasai D, Masai E, Fukuda M. 2011. Characterization of the 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE) degradation system in Janibacter sp. TYM3221. Enzyme Microb Technol. 10:532-539
  • 進藤絵里香,大坪和香子,上田裕一,上田英代,宮内啓介,遠藤銀朗. 2011. コンポスト製造過程において発生する亜酸化窒素の削減に関与する脱窒細菌遺伝子の多様性と消長. 土木学会論文集G(環境).67:III441-III448
  • 伊藤拓, 荒木直人, 遠藤銀朗, 福田雅夫, 宮内啓介. 2011. PCB分解菌における分解代謝産物存在下での分解遺伝子の転写抑制. 土木学会論文集G(環境). 67:III485-III493
  • Mei-Fang CHIEN, Ryo NAKAHATA, Tetsuya ONO, Keisuke, MIYAUCHI, Ginro ENDO. 2012. Mercury removal and recovery by immobilized mercury reducing bacterium Bacillus megaterium MB1. Frontiers of Chemical Science and Engineering. 6:192–197
  • Miyahara M., Kim S. W., Zhou S., Fushinobu S., Yamada T., Ikeda-Ohtsubo W., Watanabe A., Miyauchi K., Endo G., Wakagi T., and Shoun H.. 2012. Survival of the aerobic denitrifier Pseudomonas stutzeri strain TR2 during co-cultures with activated sludge under the denitrifying conditions. Biosci Biotech Biochem. 76:495-500
  • 遠藤銀朗,宮内啓介,簡梅芳,井上千弘,北島信行. 2012. 地震?津波被災によるヒ素汚染土壌の植物による浄化技術の開発. ケミカルエンジニヤリング. 57:437-442
  • Shimodaira J, Furusawa Y, Miyazawa Y, Kasai D, Miyauchi K, Masai E, Fukuda M. 2012. The 24-bp consensus sequence responsible for regulation of the BphS1T1 two-component system in a hybrid promoter. J Biosci Bioeng. 113:279-85.
  • Iino T, Wang Y, Miyauchi K, Kasai D, Masai E, Fujii T, Ogawa N, Fukuda M. 2012. Specific Gene Responses of Rhodococcus jostii RHA1 during Growth in Soil. Appl Environ Microbiol. 78:6954-62.
  • Ikeda-Ohtsubo W, Miyahara M, Kim SW, Yamada T, Matsuoka M, Watanabe A, Fushinobu S, Wakagi T, Shoun H, Miyauchi K, Endo G. 2013. Bioaugmentation of a wastewater bioreactor system with the nitrous oxide-reducing denitrifier Pseudomonas stutzeri strain TR2. J Biosci Bioeng. 115:37-42.
  • Iino T, Miyauchi K, Kasai D, Masai E, Fukuda M. 2013. Characterization of nitrate and nitrite utilization system in Rhodococcus jostii RHA1. J Biosci Bioeng. 115:600-6
  • Thi Nguyen PA, Thi Trinh TH, Fukumitsu Y, Shimodaira J, Miyauchi K, Tokuda M, Kasai D,Masai E, Fukuda M. 2013. Gene cluster and regulation system for 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE) degradation in Janibacter sp. TYM3221. J Biosci Bioeng. 116:91-100
  • 簡梅芳、宮内啓介、井上千弘、北島信行、遠藤 銀朗. 2013. 宮城県主要河川沖積平野部の土壌ヒ素濃度と東北地方太平洋沖地震津波の影響. 土木学会論文集(G分冊) 69: 19-24
  • 簡梅芳,小畑和貴,黄毅,宮内啓介,遠藤銀朗. 2013. ヒ素高蓄積植物による土壌ヒ素の吸収?除去と土壌微生物の亜ヒ酸酸化に関する研究. 土木学会論文集G(環境). 2013. 69:III9-III15
  • 伊藤拓,遠藤銀朗,福田雅夫,宮内啓介. 2013. カテコール分解遺伝子導入によるPCB分解菌の分解遺伝子群転写活性の強化.土木学会論文集G(環境). 69:III485-III493
  • Yamada T, Araki S, Ikeda-Ohtsubo W, Okamura K, Hiraishi A, Ueda H, Ueda Y, Miyauchi K, Endo G. 2013. Community structure and population dynamics of ammonia oxidizers in composting processes of ammonia-rich livestock waste. Syst Appl Microbiol. 2013. 36:359-67
  • Ikeda-Ohtsubo W., Miyahara M., Yamada T., Watanabe A., Fushinobu S., Wakagi T., Shoun H., Miyauchi K., and Endo G. 2013. Effectiveness of heat treatment to protect introduced denitrifying bacteria from eukaryotic predatory microorganisms in a pilot-scale bioreactor. J Biosci Bioeng. 116:722-724
  • 伊藤 拓,遠藤銀朗,福田雅夫,宮内啓介. 2014. PCB分解菌Rhodococcus jostii RHA1におけるBphS / BphT支配下遺伝子群の発現への中間分解産物カテコールの影響. J. Environ. Biotech. 14:57-63
  • Oyetibo GO, Ishola ST, Ikeda-Ohtsubo W, Miyauchi K, Ilori MO, Endo G. 2015. Mercury bioremoval by Yarrowia strains isolated from sediments of mercury-polluted estuarine water. Appl Microbiol Biotechnol. 99:3651-7.
  • Huang Y, Miyauchi K, Inoue C, Endo G. 2016. Development of suitable hydroponics system for phytoremediation of arsenic contaminated water using an arsenic hyperaccumulator plant Pteris vittata. Biosci Biotech Biochem. 80:614-8.
  • Oyetibo GO, Miyauchi K, Suzuki H, Ishikawa S, Endo G. 2016. Extracellular mercury sequestration by exopolymeric substances produced by Yarrowia spp.: Thermodynamics, equilibria, and kinetics studies. J Biosci Bioeng. 122:701-707
  • Oyetibo GO, Miyauchi K, Suzuki H, Endo G. 2016. Mercury removal during growth of mercury tolerant and self-aggregating Yarrowia spp. AMB Express. 6:99.
  • Huang Y, Miyauchi K, Endo G, Don LD, Manh NC, Inoue C. 2016. Arsenic contamination of groundwater and agricultural soil irrigated with the groundwater in Mekong Delta, Vietnam. Environ Earth Sci. 75:757-763.
  • Oyetibo GO, Miyauchi K, Huang Y, Chien MF, Ilori MO, Amund OO, Endo G. 2017. Biotechnological remedies for the estuarine environment polluted with heavy metals and persistent organic pollutants. Int Biodeterio Biodeg. 119:614-625.
  • 黄 毅, 宮内 啓介, 水戸 光昭, 中村 真理子, 成瀬 美樹, 遠藤 司, 井上 千弘, 遠藤 銀朗. 2018.ヒ素高蓄積植物による建設発生土処理地からのヒ素含有アルカリ性浸出水浄化方法の開発. 土木学会論文集G(環境). 74:1-7.

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査読なし論文?その他
  • Nagata, Y., Fukuda, M., Miyauchi, K., and Takagi, M. Genes and Enzymes for gamma-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26. Molecular Biology of Pseudomonads (ASM press) 58-70 (1996)
  • 福田雅夫、宮内啓介、政井英司 Rhodococcus属細菌のPCB分解システム 蛋白質核酸酵素 2005年10月号 vol.50 1541-7.
  • 宮内啓介 「責任者をあぶりだす!」生物工学会誌2006年6月号 vol.84 241
  • 宮内啓介. 2010. 欧洲冠军联赛_欧洲冠军联赛投注_官网ハ?イオテクノロシ?ー?リサーチ?コモン棟. 生物工学会誌. 88:685.
  • 宮内啓介,簡梅芳,黄毅,大友俊介,和泉卓也,井上千弘,北島信行,遠藤銀朗. 2013. 津波による土壌のヒ素汚染とその修復への取り組み. J Env Biotech. 13:27-30.
  • 宮内啓介. 2013.ヒ素耐性菌のヒ素耐性遺伝子群の機能解析とヒ素高蓄積植物を用いたヒ素汚染土壌の浄化. J Japan Soc Extremophiles. 12:50-55.
  • 宮内啓介. 2013. 東日本大震災によるヒ素汚染のファイトレメディエーション. 化学と生物. 51:515-517.
  • 宮内啓介. 2014. 植物を用いたヒ素汚染対策. 水環境学会誌. 37:405-408.
  • 遠藤銀朗, 黄毅, 宮内啓介. 2015. ヒ素汚染地下水の亜ヒ酸酸化微生物とヒ素高蓄積植物の水耕栽培による低コスト浄化技術の開発. J Environ Biotech. 5:63-71.
  • 大坪和香子,進藤絵里香,山田剛史,上田英代,上田裕一,渡辺昭,宮内啓介,遠藤銀朗. 2015. 畜産廃水処理システム由来の固形廃棄物の堆肥化における環境負荷低減化. J Env Biotech. 15:17-22.
  • 黄毅、宮内啓介、井上千弘、遠藤銀朗. 2016. ヒ素超蓄積植物を用いたひ素汚染水浄化のための水耕栽培システムの開発(翻訳論文). 欧洲冠军联赛_欧洲冠军联赛投注_官网工学部研究報告. 50:43-48.
  • 宮内啓介、黄 毅、菅原一輝、水戸光昭、成瀬美樹、中村真理子、門間聖子、井上千弘、遠藤銀朗. 2017. ヒ素高蓄積植物を用いたヒ素汚染水処理技術の開発. J Env Biotech.17:35.

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研究分野欧洲冠军联赛_欧洲冠军联赛投注_官网

[分子微生物学、環境微生物工学]

I. 環境汚染物質分解菌の分解機構の分子生物学的解析:

  • PCB分解菌Rhodococcus sp. RHA1のゲノム配列を利用した分解能強化
  • ダイオキシン分解菌Nocardioides sp. DF412の分解経路と分解遺伝子群転写制御機構の解明
  • 農薬分解菌の分解機構に関する分子生物学解析

II. 微生物を用いた物質生産

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